Analytical Methodologies for the Characterization of Metabolites of Aromatic Amines in Fish
Abstract
The research staff from the Center for Lake Superior Environmental Studies (CLSES, University of
Wisconsin-Superior) and the U.S. EPA Environmental Research Laboratory-Duluth (ERL-D) have
developed and implemented a successful aquatic organism biotransformation research program. The
efforts extended during the course of the cooperative agreement provided evidence of microsomal
N-hydroxylation of primary aromatic amines by Japanese medaka and rainbow trout. The
N-hydroxylation assay and metabolite analyses were developed at ERL-D through the cooperative
efforts of UW-S/CLSES and U.S. EPA staff. The acquired data clearly provide evidence that trout and
medaka form the proximate carcinogen, phenylhydroxylamine, from primary aromatic amines. This
knowledge can be applied in support of the ongoing medaka carcinogenicity bioassays being performed
at ERL-D because the known metabolic activation pathway (N-hydroxylation) for mammals has now been
detected in fish. The following report includes an overview of the methods employed, results
obtained over the past three years, and a discussion that incorporates relevant information
obtained from the literature.
Subject
analytical methodologies
metabolites
aromatic amines
fish
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http://digital.library.wisc.edu/1793/85754Type
Technical Report