Investigating Fyv6’s role in splicing through RNA sequencing and selective protein depletion
Abstract
Splicing of pre-mRNAs is an essential process for regulation of eukaryotic gene
expression. Splicing is carried out by the spliceosome, a highly dynamic complex. Mutations
within the spliceosome are responsible for various diseases in humans. Studies have recently
provided evidence for a novel splicing factor Fyv6 in Saccharomyces cerevisiae to be the
homologous protein to human splicing factor FAM192A1
. Our publication on Fyv6 elucidated
some of its function, however further research is necessary to fully understand Fyv6’s role within
the spliceosome. Herein I describe experiments that would aid in further exploring this splicing
factor through RNA sequencing and construction of yeast strains with an inducible degraded or
sequestered Fyv6 protein. We outline initial RNA-seq experiment results and our efforts to tag
Fyv6 in yeast for selective depletion. Through this work, we aim to further our understanding of
the protein components that form the spliceosome, and its mechanism of gene regulation.
Permanent Link
http://digital.library.wisc.edu/1793/84960Type
Thesis