The Apoptotic Role of Cpeb1 in INS-1 Cells

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Date
2022Author
Jayaraaman, Hariharan
Advisor(s)
Blum, Barak
Dex Nimkulrat, Sutichot
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The dedifferentiation of pancreatic (3-cells commonly occurs in the pathogenesis of both type 1
and type 2 diabetes. The (3-cell identity undergoes unique changes depending on the diabetogenic
stress that is encountered. One such change is the lower levels of Cpeb 1 in (3-cells affected by
both types of diabetes relative to healthy mature (3-cells. However, the cellular function(s) of
Cpeb 1 in (3-cells has not been explored. We hypothesized that the downregulation of Cpeb 1 in
INS-I cells (from a rat insulinoma cell line) will result in higher levels of apoptosis when faced
with diabetogenic stress from proinflammatory cytokines (characteristic of type I diabetes) or
high concentrations of glucose and palmitate (characteristic of type 2 diabetes). We found that
the in vitro knockdown of Cpeb I in INS-I cells results in higher levels of apoptosis when under
stress of proinflammatory cytokines but not of high glucose and palmitate concentrations.
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http://digital.library.wisc.edu/1793/84018Type
Thesis