Acute Toxicity of Lead to the Annelid Lumbriculus variegatus and the Amphibian Rana catesbiana

Abstract

Acute 96-h flow-through, toxicity tests with lead were conducted using the annelid Lumbriculus variegatus and the bullfrog Rana catesbiana as the test organisms. The toxicity tests were-conducted to generate ECSO or LCSO values at 4, 8, 24, 48, 72 and 96 h of exposure. The tests were conducted at the University of Wisconsin-Superior (UW-S) testing facilities according to procedures recommended by the American Society for Testing and Materials (ASTM 1993)•