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dc.contributor.advisorLi, Xinhui
dc.contributor.authorRecker, Jordan
dc.date.accessioned2018-12-11T17:23:14Z
dc.date.available2018-12-11T17:23:14Z
dc.date.issued2018-05
dc.identifier.urihttp://digital.library.wisc.edu/1793/78859
dc.description.abstractThis study evaluated the efficacy of copper alloy surfaces for inactivation of Tulane virus, a human norovirus surrogate, using plaque assay and porcine gastric mucin- conjugated magnetic beads (PGM-MB) binding assay followed by quantitative reverse transcription-PCR (PGM-MB/PCR assay). Additionally, the sensitivities of human norovirus GII.4 Sydney and GI.3B Potsdam strains to copper alloy surfaces were assessed using PGM-MB/PCR assay. Time-dependent inactivation of viruses on copper alloy coupons revealed that, for Tulane virus, 15 min of copper alloy surface treatments achieved more than 4-log reductions, as assessed by plaque assay, while up to 20 min of copper alloy surface treatments only achieved ~2-log reductions, as assessed by PGM- MB/PCR assay. As assessed by PGM-MB/PCR assay, 10 min of copper surface treatments achieved reductions of 3 and 4 log units for human norovirus GII.4 Sydney and GI.3B Potsdam, respectively. Results from this study suggest that even though PGM- MB/PCR assay underestimated the efficacy of copper alloy surface inactivation of Tulane virus, copper alloys could effectively inactivate Tulane virus and human noroviruses. Therefore, copper alloys can be used as a preventive measure to prevent human norovirus infection and an effective surface treatment for human noroviruses.en_US
dc.language.isoen_USen_US
dc.subjectMicrobiologyen_US
dc.subjectNorovirusen_US
dc.titleEvaluation of copper alloy surfaces for inactivation of Tulane virus, a human norovirus surrogate, and human noroviruses.en_US
dc.typeThesisen_US


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