FCGR3A Variability: Copy Number, Single Nucleotide Polymorphisms, and Efficacy of immunotherapy

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Date
2017-05-15Author
Gallenberger, Mikayla
Advisor(s)
Erbe-Gurel, Amy
Sondel, Paul
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Show full item recordAbstract
In order to develop personalized immunotherapies for diseases like cancer, a key
component is to understand the profiles of specific genes that may influence the response
to treatment. For example, genes like the Fc gamma receptors (FCGR) are subject to
genetic variations that alter the protein product, resulting in differential impact on the
degree of response to immunotherapies. Some FCGRs are copy number variable (CNV),
while others have single nucleotide polymorphisms (SNP); this combination adds
complexity to the genotyping process. We adapted reference-query pyrosequencing
(RQPS) to simultaneously genotype the SNP and CNV of FCGR3A. After further
troubleshooting, we will utilize RQPS to genotype Renal Cell Carcinoma (RCC) clinical
trial patients and delineate the relationship between their genotypes and responses to
current immunotherapy treatments in order to help clinicians decide which drugs to
prescribe to future patients.
Permanent Link
http://digital.library.wisc.edu/1793/76473Type
Thesis