The Identification and Characterization of Cytoplasmic Acetylated Lysines

File(s)
Date
2011Author
Stein, Kelsey
Department
Genetics
Advisor(s)
Westmark, Pamela
Malter, Jim
Metadata
Show full item recordAbstract
Lysine acetylation by histone acetyl transferascs (HATs) is a reversible posttranscriptional
modification that plays a key role in regulating several biological processes, such as the cell cycle, nuclear transport, and gene expression. Previous research has shown lysine acetylation contributes to regulation of almost all nuclear
functions, but a recent study in Science determined that lysine acetylation also
contributes to regulation of a large array of cytoplasmic functions. The goal of my
research is to determine the function, recognition, regulation, and activity of the
cytoplasmic acctylated lysines and the proposed isomerase that regulates them. A large number or enzymatic activity assays were completed using a synthesized acetylated peptide in the cis conformation. Isomerization, and therefore activity, was measured using absorbance on a spectrophotometer. Lysine acetylation has become an important target in cancer and neurodegcncrative diseases and understanding the underlying mechanisms of acetylation could aid in the improvement of drug therapy.
Subject
Pathology and Laboratory Medicine
Genetics
Permanent Link
http://digital.library.wisc.edu/1793/67921Type
Thesis
Description
22 p.