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dc.contributor.advisorPagliarini, David
dc.contributor.authorBarber, Grant
dc.date.accessioned2014-01-17T22:39:48Z
dc.date.available2014-01-17T22:39:48Z
dc.date.issued2012
dc.identifier.urihttp://digital.library.wisc.edu/1793/67917
dc.description24 p.en
dc.description.abstractMitochondria are chiefly tasked with the production of ATP. Reversible phosphorylation is postulated to be a central regulatory mechanism in mitochondrial biology, and yet the mitochondrial kinases that perform the phosphorylation events are largely uncharacterized[l]. One group of atypical mitochondrial kinases, the ADCKs (aarF-domain containing kinases), has garnered interest because of the role one of its members play in human disease. Here, we designed and validated a radiolabeling based approach to identify substrates of atypical mitochondrial kinases. To detect labeled proteins, we optimized SDS-PAGE; to detect labeled lipids, we optimized thin layer chromatography (TLC). By manipulating kinase reaction conditions, we reduced background phosphorylation of proteins and lipids by endogenous kinases. From there, we established a high-throughput, in vitro kinase assay to validate kinase-substrate pairs. Our next step is to use this approach to identify substrates of the ADCK family to elucidate their roles in human health and disease.en
dc.language.isoen_USen
dc.subjectBiochemistryen
dc.titleMethods for Identifying Substrates of Atypical Mitochondrial Kinasesen
dc.typeThesisen
thesis.degree.levelBSen
thesis.degree.disciplineBiochemistryen


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