| dc.contributor.advisor |
Rutledge, Jackie J. |
|
| dc.contributor.advisor |
Monson, Rick |
|
| dc.contributor.author |
Quam, Jessica |
|
| dc.date.accessioned |
2006-07-11T21:13:44Z |
|
| dc.date.available |
2006-07-11T21:13:44Z |
|
| dc.date.issued |
2004 |
|
| dc.identifier.uri |
http://digital.library.wisc.edu/1793/6695 |
|
| dc.description |
7 p. |
|
| dc.description.abstract |
Most in vitro embryo production (IVP) systems use some form of bovine sera in media. The most commonly used sera in maturation of bovine oocytes is fetal calf serum. A potential problem arising from this practice is disease transfer to the embryo as the sera is coming from the same species as the embryo. A potential substitute (white yolk from avian eggs) derived from a different order of animals could ameliorate this problem. This study was done to determine if white yolk plasma is a suitable supplement to use in the maturation of bovine embryos. Eight replications were done: a control (10% fetal calf serum), and three treatments, replacing fetal calf serum with 5%, 10% or 15% white yolk plasma. Data on fertilization, cleavage, and blastocyst development rate and post freezing/thawing survival were collected. There were no significant differences among treatments for fertilization, cleavage, blastocyst development, or post freezing/thawing survival indicating that white yolk is a suitable substitute for fetal calf serum in bovine oocyte maturation systems |
en |
| dc.description.provenance |
Submitted by Beverly Phillips (bphillips@library.wisc.edu) on 2006-07-11T21:13:23Z
No. of bitstreams: 1
2004_Quam.pdf: 658210 bytes, checksum: ed4f4a78a72d59ed6bf7a31018b92651 (MD5) |
en |
| dc.description.provenance |
Approved for entry into archive by Beverly Phillips(bphillips@library.wisc.edu) on 2006-07-11T21:13:44Z (GMT) No. of bitstreams: 1
2004_Quam.pdf: 658210 bytes, checksum: ed4f4a78a72d59ed6bf7a31018b92651 (MD5) |
en |
| dc.description.provenance |
Made available in DSpace on 2006-07-11T21:13:44Z (GMT). No. of bitstreams: 1
2004_Quam.pdf: 658210 bytes, checksum: ed4f4a78a72d59ed6bf7a31018b92651 (MD5)
Previous issue date: 2004 |
en |
| dc.format.extent |
658210 bytes |
|
| dc.format.mimetype |
application/pdf |
|
| dc.language.iso |
en |
en |
| dc.subject |
Animal Sciences |
en |
| dc.title |
Effects of white yolk concentration on maturation, fertilization, and blastocyst development of bovine embryos produced in vitro |
en |
| dc.type |
Thesis |
en |
