• Login
    View Item 
    •   MINDS@UW Home
    • MINDS@UW La Crosse
    • Murphy Library, UWL
    • UW-L Master's Theses
    • View Item
    •   MINDS@UW Home
    • MINDS@UW La Crosse
    • Murphy Library, UWL
    • UW-L Master's Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Development of microscale assays to screen for novel anthelmintic drugs

    Thumbnail
    File(s)
    OluwafadekeniAwoyinka2010.pdf (883.4Kb)
    Date
    2010-05
    Author
    Awoyinka, Oluwafadekemi
    Metadata
    Show full item record
    Abstract
    Parasitic nematodes are beginning to show resistance to the most commonly used classes of anthelmintic drugs at an alarming rate. As the number of documented cases of resistance increases, people are becoming more interested in identifying new sources of anthelmintic drugs. Since the emergence of popular anthelmintics in the 1980s, there has been little effort put into the development of new drugs for use against helminths. Recently, a few studies have investigated new sources for anthelmintics, including one that discovered a whole new class of compounds that showed anthelmintic activity against resistant nematode species. Previous studies used high volume assays which require milliliter amounts of the compounds being screened for anthelmintic abilities. The goal of this study was to develop new screening processes for identifying anthelmintic activity in naturally derived compounds using microliter volumes of the test compound solutions. Two microscale assays were developed using the nematode Caenorhabditis elegans as a model system: one looking for the immediate effects on motility and the other investigating the effects of each compound on development and fecundity of the worms. The assays developed in this project were used to identify several compounds of interest that could be further investigated as potential anthelmintic drugs.
    Subject
    Effect of drugs on.
    Caenorhabditis elegans.
    Drug resistance.
    Anthelmintics.
    Nematoda.
    Permanent Link
    http://digital.library.wisc.edu/1793/54703
    Part of
    • UW-L Master's Theses

    Contact Us | Send Feedback
     

     

    Browse

    All of MINDS@UWCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Contact Us | Send Feedback