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dc.contributor.advisorWeaver, Todd
dc.contributor.advisorSanderfoot, Anton
dc.contributor.advisorCooper, Scott
dc.contributor.advisorHoffman, Michael
dc.contributor.authorHulseberg, Christine
dc.date.accessioned2011-06-07T18:27:28Z
dc.date.available2011-06-07T18:27:28Z
dc.date.issued2011-02-23
dc.identifier.urihttp://digital.library.wisc.edu/1793/53278
dc.description.abstractHuman parainfluenza virus type 3 (HPIV3) is a major cause of bronchiolitis and pneumonia in infants under 6 months of age. Like other enveloped RNA viruses, HPIV3 encodes a matrix (M) protein involved in the final assembly and budding steps of the viral life cycle. Illustrating the important role of M protein is its ability to induce its own budding from cells in the form of enveloped virus-like particles (VLPs). For related viruses, the feature of viral M proteins that allows this independent budding to occur is a critical amino acid sequence, called a late (L) domain, which interacts with the host cell vesicle-forming machinery. To identify the HPIV3 L domains, we selected four HPIV3 M protein sequences (PPKH, YLDV, KPEL, and YPNI) based on their sequence similarity to established L domains and made alanine-substitution mutants of each potential L domain sequence. When these mutant M proteins were expressed in cells, we found that disrupting the YLDV sequence caused a severe budding defect. To confirm these results, we then inserted these sequences into poorly budding L domain-deficient mutants of the Ebola virus matrix protein. Consistent with our previous findings, the YLDV-containing VP40-delta N13 mutant was able to restore budding efficiency to levels on par with wild-type VP40. These findings provide strong evidence to support the likelihood that the YLDV sequence of HPIV3 M protein functions as an L domain.en
dc.language.isoen_USen
dc.subjectVirus diseases in childrenen
dc.subjectExtracellular matrix proteins -- Physiological effecten
dc.subjectParainflenza virusesen
dc.titleIdentification of a novel late domain in human parainfluenza virus type 3 matrix proteinen
dc.typeThesisen
thesis.degree.levelMSen
thesis.degree.disciplineClinical Microbiologyen


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