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Identifying and locating length and position of introgression on chromosome 6 influencing resistance to bacterial wilt and identifying markers for bacterial wilt resistance on chromosome 12

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Ewert, Eric Robert
Maxwell, Douglas (Mentor)
BS, Biology
Plant Pathology; Biology
Bacterial wilt is a devastating disease affecting hundreds of species of plants and millions of people by destroying crops like the tomato. Conventional techniques to destroy the pathogen have been futile to this point and breeding for genetic resistance to the disease appears to be the most effective option. Using tomato germplasm field tested for resistance and susceptibility in Guatemala, PCR techniques and gel electrophoresis were used to obtain DNA fragments from specific chromosome regions believed to be important in resistance. These DNA fragments were then sequenced, and the sequences were compared from the susceptible and resistant germplasm. During this process, two separate introgressions were identified, where susceptible and resistant cultivars were different in sequences. Introgressions were located at 41.5 cM on chromosome 6 and 39 cM on chromosome 12. These introgressions were also associated with other resistant and susceptible germplasm, which had been evaluated in the field in Guatemala. The specific single nucleotide polymorphism associated with the resistant germplasm could be used in breeding programs employing marker-assisted selection methods.
27 p.
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