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dc.contributor.advisorKim, Kyung Won (Mentor)
dc.contributor.advisorKimble, Judith (Mentor)
dc.contributor.authorWilson, Tracy
dc.date.accessioned2010-07-21T17:41:37Z
dc.date.available2010-07-21T17:41:37Z
dc.date.issued2010
dc.identifier.urihttp://digital.library.wisc.edu/1793/46048
dc.description29 p.en
dc.description.abstractCytoplasmic polyadenylation is a key mechanism of gene regulation in all eukaryotes. The C. elegans GLD-2 protein provides the catalytic activity for a cytoplasmic poly(A) polymerase, but GLD-2 must partner with another protein that provides RNA-binding activity to produce a fully functional enzyme. One such GLD-2 binding partner is RNP-8, which contains an RNA recognition motif. Here, we explored the RNA-binding specificity of RNP-8. Using the yeast-three hybrid system, we identified 34 unique RNA sequences that were bound by RNP-8, but we could not identify a consensus sequence among the 34 sequences. Therefore, RNP-I protein may lack sequence specificity when binding to RNA.en
dc.language.isoen_USen
dc.rightsThe author grants to the University of Wisconsin-Madison the permission to reproduce and to distribute publicly paper and electronic copies of this thesis document in whole or in part in any medium now known or hereafter created.en
dc.subjectBiochemistryen
dc.subjectBiologyen
dc.titleAttempts to analyze the RNA-binding specificity of the C. elegans RNP-8 proteinen
dc.typeThesisen
thesis.degree.levelBSen


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