• Login
    View Item 
    •   MINDS@UW Home
    • MINDS@UW Madison
    • College of Agricultural and Life Sciences, UW-Madison
    • College of Agricultural and Life Sciences Honors and Undergraduate Research Program
    • Biology Honors Theses and Research Papers
    • View Item
    •   MINDS@UW Home
    • MINDS@UW Madison
    • College of Agricultural and Life Sciences, UW-Madison
    • College of Agricultural and Life Sciences Honors and Undergraduate Research Program
    • Biology Honors Theses and Research Papers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    LEF/TCF family transcription factors are important in the upregulation and restricted expression of zic genes in the dorsal neural tube

    Thumbnail
    File(s)
    2009_Webert.pdf (862.8Kb)
    Date
    2009
    Author
    Webert, Kyle C.
    Advisor(s)
    Grinblat, Yevgenya
    Metadata
    Show full item record
    Abstract
    A central question in biology is how different cell-types arise from an initially one-celled embryo to form an organism. Many cellular differences arise through differential gene expression in space and time via the action of transcription factors (TFs). Zic genes are TFs that contribute to the patterning of the developing vertebrate nervous system, but little is known about how their transcription is regulated. Consensus scoring across several vertebrate species identified possible loci for TF binding sites within a previously identified stretch of zic regulatory DNA, cis-regulatory module D5. The three most promising sites were mutated and injected in constructs upstream of eGFP reporters into zebrafish embryos at the one cell stage. Expression was observed in F1 transgenics by in vivo fluorescence at approximately 24 hrs. The mutation of the LEF TF binding site suggests that Zic expression is upregulated and restricted to the dorsal neural tube by action of LEF TFs.
    Subject
    Biology
    Zoology
    Permanent Link
    http://digital.library.wisc.edu/1793/37514
    Description
    14 p.
    Part of
    • Biology Honors Theses and Research Papers

    Contact Us | Send Feedback
     

     

    Browse

    All of MINDS@UWCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Contact Us | Send Feedback