• Login
    View Item 
    •   MINDS@UW Home
    • MINDS@UW Madison
    • College of Agricultural and Life Sciences, UW-Madison
    • College of Agricultural and Life Sciences Honors and Undergraduate Research Program
    • Biochemistry Honors Theses and Research Papers
    • View Item
    •   MINDS@UW Home
    • MINDS@UW Madison
    • College of Agricultural and Life Sciences, UW-Madison
    • College of Agricultural and Life Sciences Honors and Undergraduate Research Program
    • Biochemistry Honors Theses and Research Papers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Chronic and acute seizure activity affects NKCC1 and KCC2 expression and alters GABA-induced inhibition in the adult and developing hippocampus

    Thumbnail
    File(s)
    2008_Gielissen.pdf (890.1Kb)
    Date
    2008
    Author
    Gielissen, Katherine A.
    Advisor(s)
    Sutula, Thomas P.
    Metadata
    Show full item record
    Abstract
    Temporal lobe epilepsy is a relatively common nervous system disorder characterized by abnormal activity in the hippocampus, often caused by a decrease in GABA inhibition. GABAA receptors are Cl- ion channels whose effects are medicated by inward Cl- rectifying (excitatory) NKCC1 and outward Cl- rectifying (inhibitory) KCC2 cotransporters. The expression of these cotransporters is modified by both aberrant neural activity and "critical periods" in development. We hypothesized the hippocampi class V seizures would cause a permanent upregulation of NKCC1 and/or downregulation of KCC2. We also hypothesized rats with seizures during postnatal days 20-30 would cause long term dysfunction in hippocampal expression of these cotransporters. Western blotting results suggest that increased propensity for seizures is due to permanent changes in NKCC1 levels, which subsequently reduces GABAA-mediated inhibition. Furthermore, developing rats that experience a seizure at P25 display decreased KCC2 expression levels both 24 hours and 90 days after the event, with little change in NKCC1 expression?with similar physiological effects as in adults.
    Subject
    Neurology
    Biochemistry
    Permanent Link
    http://digital.library.wisc.edu/1793/37484
    Description
    23 p.
    Part of
    • Biochemistry Honors Theses and Research Papers

    Contact Us | Send Feedback
     

     

    Browse

    All of MINDS@UWCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Contact Us | Send Feedback