Magnetic resonance microscopy of flows and compressions of the circulatory, respiratory, and digestive systems in pupae of the tobacco hornworm, Manduca sexta
Each frame is separated by 316 ms. Movie 1 corresponds to Figure 1A-C. See Figure 1A for orientation and structural information. Hemolymph flow through the dorsal vessel and ventral diaphragm is seen throughout the movie. (2.295Mb)
Each frame is separated by 316 ms. Movie 2 corresponds to Figure 1D–F. See Figure 1G for orientation and structural information and the text for a detailed description. Several pulses of hemolymph flowing through the dorsal vessel are observed on the right side of the movie, which is the dorsal side of the pupa. (1.343Mb)
Each frame is separated by 316 ms. Movie 3 corresponds to Figure 3A-D. See Figure 3A for orientation and structural information and the text for a detailed description. Two cycles of compression and inflation of the air sacs are seen about halfway through the movie. (2.969Mb)
Each frame is separated by 316 ms. Movie 4 corresponds to Figure 3E–H. See Figure 3E for orientation and structural information and the text for a detailed description. Several cycles of compression and inflation of the air sacs as well as abdominal motion are seen during the first half of the movie. (2.807Mb)
Hallock, Kevin J.
Journal of Insect Science
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Circulatory, respiratory, and digestive motions in Manduca sexta pupae were observed using proton-density weighted and fast-imaging with steady-state free procession magnetic resonance microscopy. Proton-density weighted images clearly differentiated pupal air sacs from the hemolymph and organs because, as expected, the air sacs appeared dark in these images. Steady-state free procession imaging allowed real-time monitoring of respiration and circulation, creating movies of hemolymph circulation. Some of the movies show compression and inflation of the air sacs as well as abdominal movements consistent with previously reported ceolopulses. To our knowledge, this is the first magnetic resonance microscopy study of insect circulation and respiration and these preliminary results demonstrate the potential of magnetic resonance microscopy for studying in vivo dynamic processes in insects.
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