• Login
    View Item 
    •   MINDS@UW Home
    • MINDS@UW Eau Claire
    • UWEC Office of Research and Sponsored Programs
    • Student Research Day
    • View Item
    •   MINDS@UW Home
    • MINDS@UW Eau Claire
    • UWEC Office of Research and Sponsored Programs
    • Student Research Day
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Getting COSY with Methanobactin : NMR chromopeptide characterization

    Thumbnail
    File(s)
    ScardinoSpr07.pdf (2.446Mb)
    Date
    2007-05-01
    Author
    Behling, Lee A.
    Scardino, Lori L.
    Rao, Vinay B.
    Advisor(s)
    Hartsel, Scott C.
    Gallagher, Warren H.
    Dispirito, Alan A.
    Metadata
    Show full item record
    Abstract
    Methanobactin (mb) is a chalkophore produced by Methylosinus trichosporium OB3b. This non-ribosomal chromopeptide is a part of the copper acquisition system of these methane oxidizing bacteria. We have obtained NMR spectra of mb after titration with Cu(II). This is only possible because mb reduces Cu(II) to Cu(I) upon binding and thus becomes diamagnetic and slightly less soluble. We have not yet identified the specific reductant which is also capable of reducing Hg(II), Ag(I) and Au(III). Cu(I) in solution is typically unstable; however, it remains stable in solution when bound to mb. Mb is thought to bind metal ions using nitrogen and sulfur ligands from its 4-thiocarbonyl-5-hydroxy imidazole (THI), 4-hydroxy-5-thiocarbonyl imidazole (HTI) and possibly tyrosine, methionine and other residues. Our experiments show that there are considerable changes in their environments denoted by significant changes in the proton NMR spectra of mb when it is bound to copper versus copper-free mb. Residue assignments have been made using COSY and TOCSY measurements. Protons on the nitrogens of HTI and THI have been identified using 15N NMR.
    Subject
    Methanobactin
    Posters
    Permanent Link
    http://digital.library.wisc.edu/1793/23175
    Description
    Color poster with text, graphs and charts describing research conducted by Lori L. Scardino, Vinay B. Rao, and Lee A. Behling advised by Scott C. Hartsel, Warren H. Gallagher, and Alan A. Dispirito.
    Part of
    • Student Research Day

    Contact Us | Send Feedback
     

     

    Browse

    All of MINDS@UWCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Contact Us | Send Feedback