Browsing Biochemistry Honors Theses and Research Papers by Title
Now showing items 69-84 of 84
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The RecA-dependent endonuclease Ref: characterization of DNA binding, nuclease activity, and use for in vivo genome editing in Escherichia coli
(2014)Bacteriophage Ref protein is an HNH class, RecA-dependent endonuclease. RecA-bound oligonucleotides complementary to a region on circular dsDNA will catalyze RecA strand-invasion, creating a displacement loop (D-loop). ... -
Regions of functional upstream domain of protein F1 of streptococcus pyogenes necessary for exposure of tenth type III module in fibronectin
(2010)The 49 amino acid portion of the F1 surface protein of Streptococcus pyogenes known as the functional upstream domain (FUD) interacts with Fn in an unknown manner that prevents assembly of a Fn matrix. When FUD binds to ... -
A role for FoxQ-1 in memory and learning
(2010)Using a genome-wide screen, genes that were differentially expressed between aged rats that were able to learn a spatial task (aged superior learners: SL), and learning-impaired rates (AI) were identified in the CA1 region ... -
Roles for the ESCRT Machinery and the Septin Complex in Membrane Abscission During Cytokinesis
(2015)Abscission occurs during the final phase of cytokinesis, effectively partitioning a mother cell into two separate daughter cells. The process involves the scission of the membranous intracellular bridge, resulting in the ... -
Second Sphere Effects of O2 Activation in Stearoyl-Acyl Carrier Protein Delta9 Desaturase
(2008)Stearoyl-acyl carrier protein Delta9 desaturase (Delta9D) from Ricinus communis converts stearic acid into monounsaturated oleic acid and functions to maintain the lipid composition of the cell membrane. While the desaturase ... -
Stable expression and purification of Juvenile Hormone Binding Protein from Drosophila melanogaster Schneider line-2 cells
(2007)Studies on the hemolymph Juvenile Hormone Binding Protein (hJHBP) and Juvenile Hormone (JH) interaction have been considerably limited by the availability of pure hJHBP. An expression vector was created by cloning the ... -
Steam distilled extract of ginger is a potent mediator of apoptosis in endometrial cancer cells via the modulation of the p53 and BcI-2 pathways
(2012)Development for new therapeutic and preventative agents in the treatment of gynecological cancers is in constant need. In the cunent study, we demonstrate that the steam distilled extracts of ginger are potent mediators ... -
Synthesis and Characterization of Gamma-Peptide Foldamers
(2011)Elucidating folding properties of unnatural amino acids has enabled the start of function-directed design. Two types of gamma-amino acids are probed for their abilities to fold into helical secondary structure. Both ... -
Synthesis of substrates for the galactofuranosyl transferase GlfT2
(2011)The galactan, a polymer of galactofuranose (Galf), is a key component of the hydrophobic cell wall of Mycobacterium tuberculosis, the bacterial species that is responsible for tuberculosis. Much remains unknown about the ... -
Tanscriptome comparison of ionizing radiation resistant strain of Escherichia coli, CB2000, and naturally occurring strain of Escherichia coli, Founder
(2007)CB2000 is a strain of Escherichia coli (E. coli) that is extremely resistant to ionizing radiation. It was derived from a colony, referred to as the founder strain, isolated from naturally occurring Escherichia coli K12 ... -
A Targeted RNAi Screen Identifies Novel Regulators of Synapse Formation and Function
(2015)An intricate knowledge of synapse formation and regulation is crucial to understanding how neurons develop, interact, and grow. In an attempt to gain a deeper comprehension of synapse development, we performed a three-part ... -
Testicular degeneration in a rat model expressing p.R106C TFG
(2021)Despite a majority of proteins depending on the early secretory pathway for export, the precise role of this machinery in development and maintenance of tissue architecture is largely unknown. The p.R106C mutation in ... -
Testing a putative species-independent translation enhancer: "3 Hairpin" motif
(2010)Cell-free (CF) protein synthesis enables rapid production and engineering of recombinant proteins. Existing CF systems differ substantially from each other with respect to efficiency and scalability. We examined the ... -
Translational regulation using the PUF scaffold and deadenylases
(2010)Translation is a key point of regulation in gene expression. A number of regulatory proteins involved in the processes of activation and repression have been characterized through tethered function assays. We designed ...