Browsing Biochemistry Honors Theses and Research Papers by Title
Now showing items 61-80 of 84
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Optimization of recombinant E. coli protein expression in growth medium
(2007)With the onset of novel genomic sequence data, much effort is now being taken to discover the role of many genes and the corresponding proteins that remain unknown. One of the limiting steps in such efforts has been the ... -
Pleiotropic drug resistance activation via over expression of Zuo1
(2010)Pleiotropic drug resistance (PDR) is the resistance to multiple drugs that occurs by upregulating gene expression of ABC (ATP-binding cassette) transporters that transport drug out of the cell. Thakur et al. (2008) ... -
Polyamide-chlorambucil conjugates
(2008)Polyamides are chemically engineered molecules made up of sequences of pyrolle and imidazole rings. In particular sequences, these molecules have been shown to have a very high affinity and specificity for nucleic acids, ... -
Pre-steady state product formation rates for mutant and wild type toluene 4-monooxygenases
(2008)This study measures the pre-steady state product formation rate for toluene 4-monooxygenase (T4MO). T4MO catalyzes the NADH and O2 dependent hydroxylation of toluene to form p-cresol. By using alternative substrates with ... -
Predicting and Interpreting the Hofmeister Effects of Different Salts with Nucleic Bases and Aromatic Compounds Using Solubility Assay
(2017-06-13)The interaction between salts and nucleic acid bases or aromatic compounds plays an important role in noncovalent biopolymer assembly processes such as DNA and RNA helix formation and protein-nucleic acid interactions. The ... -
Probing the chemistry in the active site of P4H
(2009)Prolyl 4-hydroxylase (P4H), a mononuclear, non-heme FeII, 2-oxoglutarate and O2 dependent enzyme, plays an integral role in the biosynthesis of collagen. It catalyzes the post-translational hydroxylation of proline residues ... -
Production of Clostridium thermocellum cohesion 3 fusion proteins for cell surface docking on recombinant Saccharomyces cerevisiae
(2010)Consolidated bioprocessing (CBP) for the production of bioethanol aims to harness engineered microbes capable of saccharifying and fermenting waste lignocellulosic biomass in one industrial step. With the goal of collaboratively ... -
Purification and Characterization of Human ISDll
(2014-11)Iron-Sulfur clusters, a class of cofactor used in all domains of life, play crucial roles in several physiological processes. Although many proteins of the human isc Fe-5 biosynthesis pathway have been identified, many of ... -
The RecA-dependent endonuclease Ref: characterization of DNA binding, nuclease activity, and use for in vivo genome editing in Escherichia coli
(2014)Bacteriophage Ref protein is an HNH class, RecA-dependent endonuclease. RecA-bound oligonucleotides complementary to a region on circular dsDNA will catalyze RecA strand-invasion, creating a displacement loop (D-loop). ... -
Regions of functional upstream domain of protein F1 of streptococcus pyogenes necessary for exposure of tenth type III module in fibronectin
(2010)The 49 amino acid portion of the F1 surface protein of Streptococcus pyogenes known as the functional upstream domain (FUD) interacts with Fn in an unknown manner that prevents assembly of a Fn matrix. When FUD binds to ... -
A role for FoxQ-1 in memory and learning
(2010)Using a genome-wide screen, genes that were differentially expressed between aged rats that were able to learn a spatial task (aged superior learners: SL), and learning-impaired rates (AI) were identified in the CA1 region ... -
Roles for the ESCRT Machinery and the Septin Complex in Membrane Abscission During Cytokinesis
(2015)Abscission occurs during the final phase of cytokinesis, effectively partitioning a mother cell into two separate daughter cells. The process involves the scission of the membranous intracellular bridge, resulting in the ... -
Second Sphere Effects of O2 Activation in Stearoyl-Acyl Carrier Protein Delta9 Desaturase
(2008)Stearoyl-acyl carrier protein Delta9 desaturase (Delta9D) from Ricinus communis converts stearic acid into monounsaturated oleic acid and functions to maintain the lipid composition of the cell membrane. While the desaturase ... -
Stable expression and purification of Juvenile Hormone Binding Protein from Drosophila melanogaster Schneider line-2 cells
(2007)Studies on the hemolymph Juvenile Hormone Binding Protein (hJHBP) and Juvenile Hormone (JH) interaction have been considerably limited by the availability of pure hJHBP. An expression vector was created by cloning the ... -
Steam distilled extract of ginger is a potent mediator of apoptosis in endometrial cancer cells via the modulation of the p53 and BcI-2 pathways
(2012)Development for new therapeutic and preventative agents in the treatment of gynecological cancers is in constant need. In the cunent study, we demonstrate that the steam distilled extracts of ginger are potent mediators ... -
Synthesis and Characterization of Gamma-Peptide Foldamers
(2011)Elucidating folding properties of unnatural amino acids has enabled the start of function-directed design. Two types of gamma-amino acids are probed for their abilities to fold into helical secondary structure. Both ... -
Synthesis of substrates for the galactofuranosyl transferase GlfT2
(2011)The galactan, a polymer of galactofuranose (Galf), is a key component of the hydrophobic cell wall of Mycobacterium tuberculosis, the bacterial species that is responsible for tuberculosis. Much remains unknown about the ... -
Tanscriptome comparison of ionizing radiation resistant strain of Escherichia coli, CB2000, and naturally occurring strain of Escherichia coli, Founder
(2007)CB2000 is a strain of Escherichia coli (E. coli) that is extremely resistant to ionizing radiation. It was derived from a colony, referred to as the founder strain, isolated from naturally occurring Escherichia coli K12 ...