Browsing Biochemistry Honors Theses and Research Papers by Title
Now showing items 52-71 of 84
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Large scale production of stable hemolymph juvenile hormone binding protein
(2009)To better understand the pathways of the insect juvenile hormone (JH) action, milligram amounts of pure hemolymph juvenile hormone binding protein (hJHBP) are required. Hence the goal of this study is to scale up the system ... -
Measurement of The ADCK3 Transmembrane Helix Self-Association In Escherichia Coli Via the TOXCAT Assay
(2014)The interaction of a-helices in the transmembrane region of certain proteins is important for the function of these proteins. The goal of our project is to study the self-association propensity of the transmembrane region ... -
Methods for Identifying Substrates of Atypical Mitochondrial Kinases
(2012)Mitochondria are chiefly tasked with the production of ATP. Reversible phosphorylation is postulated to be a central regulatory mechanism in mitochondrial biology, and yet the mitochondrial kinases that perform the ... -
MHC class I allele characterization in Indonesian cynomolgus macaques
(2007)There has recently been increased interest in the use of cynomolgus macaques for the study of infectious diseases. However, little focus has been given to studying the immunogenetics of these animals. To improve researcher’s ... -
Mid-life dementia risk scores as a predictor of CSF biomarker and cognitive response to simvastatin therapy in asymptomatic adults at risk for Alzheimer's disease: The ESPRIT trial
(2010)BACKGROOUND: Cardiovascular risk factors at mid-life are associated with an increased risk of developing Alzheimer's disease (AD). OBJECTIVE: Determine relationships between baseline dementia risk, cognitive function, CSF ... -
Minicircle-Derived scn8ab Kock-In Reporter Line Provides a Model to Study Neuronal Regulation of Zebrafish Fin Regeneration
(2022)Understanding the cellular and molecular mechanisms of regenerative zebrafish will advance fundamental knowledge of tissue regeneration. Our previous study identified scn8ab, which encodes a voltage-gated sodium channel, ... -
Mutations in CNGC2 in Arabidopsis thaliana lead to a reduction in fertility due to a sporophytic defect
(2008)While cyclic nucleotide gated channels (CNGCs) are known to play an important role in sensory transduction in animals, little is known about their functions and the mechanisms by which they act in plants. cngc2 plants ... -
Neuronal Ensemble Reactivation in the CA3 Hippocampal Region in Memory Formation due to Pattern Completion
(2017-06-13)The hippocampus functions in many memory processes; not only is it essential to the storage of new memories, but the retrieval of associated memories as well. A postulate of memory recollection called pattern completion, ... -
Novel insight into the patterns of in vitro short abortive RNA release by Escherichia coli RNA polymerase
(2020-05)E. coli RNA polymerase (RNAP) catalyzes prokaryotic transcription with the σ70 specificity factor, which directs the binding of RNAP to promoter DNA. RNAP operates on the promoter to form an open complex. In transcription ... -
Optimization of recombinant E. coli protein expression in growth medium
(2007)With the onset of novel genomic sequence data, much effort is now being taken to discover the role of many genes and the corresponding proteins that remain unknown. One of the limiting steps in such efforts has been the ... -
Pleiotropic drug resistance activation via over expression of Zuo1
(2010)Pleiotropic drug resistance (PDR) is the resistance to multiple drugs that occurs by upregulating gene expression of ABC (ATP-binding cassette) transporters that transport drug out of the cell. Thakur et al. (2008) ... -
Polyamide-chlorambucil conjugates
(2008)Polyamides are chemically engineered molecules made up of sequences of pyrolle and imidazole rings. In particular sequences, these molecules have been shown to have a very high affinity and specificity for nucleic acids, ... -
Pre-steady state product formation rates for mutant and wild type toluene 4-monooxygenases
(2008)This study measures the pre-steady state product formation rate for toluene 4-monooxygenase (T4MO). T4MO catalyzes the NADH and O2 dependent hydroxylation of toluene to form p-cresol. By using alternative substrates with ... -
Predicting and Interpreting the Hofmeister Effects of Different Salts with Nucleic Bases and Aromatic Compounds Using Solubility Assay
(2017-06-13)The interaction between salts and nucleic acid bases or aromatic compounds plays an important role in noncovalent biopolymer assembly processes such as DNA and RNA helix formation and protein-nucleic acid interactions. The ... -
Probing the chemistry in the active site of P4H
(2009)Prolyl 4-hydroxylase (P4H), a mononuclear, non-heme FeII, 2-oxoglutarate and O2 dependent enzyme, plays an integral role in the biosynthesis of collagen. It catalyzes the post-translational hydroxylation of proline residues ... -
Production of Clostridium thermocellum cohesion 3 fusion proteins for cell surface docking on recombinant Saccharomyces cerevisiae
(2010)Consolidated bioprocessing (CBP) for the production of bioethanol aims to harness engineered microbes capable of saccharifying and fermenting waste lignocellulosic biomass in one industrial step. With the goal of collaboratively ... -
Purification and Characterization of Human ISDll
(2014-11)Iron-Sulfur clusters, a class of cofactor used in all domains of life, play crucial roles in several physiological processes. Although many proteins of the human isc Fe-5 biosynthesis pathway have been identified, many of ... -
The RecA-dependent endonuclease Ref: characterization of DNA binding, nuclease activity, and use for in vivo genome editing in Escherichia coli
(2014)Bacteriophage Ref protein is an HNH class, RecA-dependent endonuclease. RecA-bound oligonucleotides complementary to a region on circular dsDNA will catalyze RecA strand-invasion, creating a displacement loop (D-loop). ... -
Regions of functional upstream domain of protein F1 of streptococcus pyogenes necessary for exposure of tenth type III module in fibronectin
(2010)The 49 amino acid portion of the F1 surface protein of Streptococcus pyogenes known as the functional upstream domain (FUD) interacts with Fn in an unknown manner that prevents assembly of a Fn matrix. When FUD binds to ... -
A role for FoxQ-1 in memory and learning
(2010)Using a genome-wide screen, genes that were differentially expressed between aged rats that were able to learn a spatial task (aged superior learners: SL), and learning-impaired rates (AI) were identified in the CA1 region ...