Bioassay-guided purification and characterization of anti-inflammatory components in Cinnamomum burmannii

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Author(s): Chan, Lok Yan
Advisor(s): Parkin, Kirk (Mentor)
Degree: BS, Biochemistry
Date: 2010
Subject(s): Food Science; Biochemistry
Abstract: Excessive inflammatory response is implicated in many diseases such as arthritis, atherosclerosis, and cardiomyopathy. Previous studies have found that several cinnamon species could exhibit anti-inflammatory activities. Adopting the bioassay-guided purification technique, trans-cinnamaldehyde was identified to be the principle active component in Cinnamomum burmannii. At a dosage of 50 muM, trans-cinnamaldehyde could inhibit 50% production of nitric oxide by down-regulating the expression of iNOS. It also inhibited 75% of prostaglandin E2 (PGE2) production without changing cyclooxygenase-2 (COX-2) protein expression. It was concluded that trans-cinnamaldehyde was the major component in C. burmannii responsible for its anti-inflammatory activity through inhibition of NO and PGE2 production.
Description: 13 p.
Permanent link: http://digital.library.wisc.edu/1793/38592
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