Inflammatory response and extracellular matrix turnover following pulsed dyed laser treatment of vocal fold and vocal fold fibroblasts

File(s):

Author(s): Ya, Lin
Advisor(s): Welham, Nathan V.
Date: 2008
Subject(s): Surgery; Biochemistry
Abstract: The 585-nm pulsed dye laser (PDL) is commonly utilized for the treatment of vascular and epithelial vocal fold lesions; however there are anecdotal reports that it may improve scar outcomes, perhaps by altering extracellular matrix (ECM) turnover; in certain patients. The purpose of this study was to evaluate the effects of PDL treatment on a selected group of inflammatory and procollagen/collagenase genes in normal rat vocal folds and vocal fold fibroblast cells. Vocal folds and fibroblasts were treated with 5-50J/cm2 PDL fluence and harvested at several time points. The mRNA expression profiles of TGF-Beta1, COX-2, IL-1Beta, IL-6, MMP-13, Procollagen I and Procollagen III were examined using real time RT-PCR. Our results indicated changes in inflammatory response alongside alterations in procollagen/collagenase expression, suggesting that PDL treatment does alter gene expression of fibroblasts within the focal fold ECM.
Description: 1 p. (abstract only). YOU CANNOT OPEN THE COMPLETE PAPER. It is not available to the public, in accordance with the author's wishes.
Permanent link: http://digital.library.wisc.edu/1793/37668
Export: Export to RefWorks