About This Item

Ask the MINDS@UW Librarian

Neuronal lysosome permeabilization dynamics in ischemia

Show full item record

File(s):

Author(s)
He, Shuhan
Advisor(s)
Lipton, Peter
Date
2009
Subject(s)
Biology; Physiology
Abstract
The mechanism in which lysosomes are damaged by free radicals during neuronal ischemia is examined in rat hippocampus. There are two hypotheses. The free radicals can degrade the lysosome membrane through liquefaction; all of the intralysosomal proteins would be able to penetrate the membrane, albeit at slower rates for larger proteins. The second mechanism is that pores are created; the proteins within the lysosome that are smaller than the pores can escape while those larger than the pores would not due to the robust nature of the undamaged membrane. Acid Phosphatase (67kda) and Beta-Galactosidase (540 kDa) has been identified as rat intralysosomal neuronal proteins that can be detected as proxies for leakage. Our initial results indicate that the damage undergoes the pore mechanism of damage based on preferential acid phosphatase leakage.
Description
16 p.
Permanent link
http://digital.library.wisc.edu/1793/37516 
Export
Export to RefWorks 
‚Äč

Part of

Show full item record

Search and browse




About MINDS@UW

Deposit materials

  1. Register to deposit in MINDS@UW
  2. Need deposit privileges? Contact us.
  3. Already registered? Have deposit privileges? Deposit materials.