About This Item

Ask the MINDS@UW Librarian

Characterization of Tau Phosphorylation During the Differentiation of Human Embryonic Stem Cells

Show full item record

File(s):

Author(s)
Kaltcheva, Maria
Advisor(s)
Atwood, Craig
Date
2008
Subject(s)
Biochemistry; Medicine
Abstract
Alzheimer's disease is the seventh leading cause of death in the United States with an estimated cost of 100 billion dollars. The severity of this neurodegenerative disease is strongly correlated with the number of neurofibrilary tangles (NFT) composed of hyperphosphorylated tau, a microtubule associated protein. The phosphorylation of tau is known to be tightly regulated by glycogen synthase kinase 3Beta (GSK-3Beta) and cyclin-dependent kinase 5 (Cdk-5). These two kinases also play a vital role in neuronal cell development. We characterized the phosphorylation of tau and expression of tau, GSK-3Beta, and Cdk-5 during early embryo development using human embryonic stem cells (hESC) as our model system. We determined that GSK-3Beta plays an important role in the regulation of proliferation, tau phosphorylation, and neuroectodermal differentiation of hESC. However, the inhibition of Cdk-5 did not significantly change hESC proliferation and the expression of tau, GSK-3Beta, and Cdk-5 throughout hESC differentiation.
Description
30 p.
Permanent link
http://digital.library.wisc.edu/1793/37488 
Export
Export to RefWorks 
‚Äč

Part of

Show full item record

Search and browse




About MINDS@UW

Deposit materials

  1. Register to deposit in MINDS@UW
  2. Need deposit privileges? Contact us.
  3. Already registered? Have deposit privileges? Deposit materials.