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Detection of the bacterium, Xylella fastidiosa, in saliva of glassy-winged sharpshooter, Homalodisca vitripennis

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i1536-2442-8-Ramirez-v01.mov 17.26Mb video/quicktime View/Open
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dc.contributor.author Ramirez, Jose L.
dc.contributor.author Lacava, Paulo T.
dc.contributor.author Miller, Thomas A.
dc.date.accessioned 2008-04-07T14:27:48Z
dc.date.available 2008-04-07T14:27:48Z
dc.date.issued 2008
dc.identifier.citation Ramirez, Jose L.; Lacava, Paulo T., and Thomas A. Miller. Detection of the bacterium, Xylella fastidiosa, in saliva of glassy-winged sharpshooter, Homalodisca vitripennis. Journal of Insect Science en
dc.identifier.uri http://digital.library.wisc.edu/1793/24469
dc.description.abstract Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), the glassy-winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei (Xanthomonadales: Xanthomonadaceae) that causes Pierce’s Disease in grapevines in California. In the present study we report a new method for studying pathogen transmission or probing behavior of H. vitripennis. When confined, H. vitripennis attempt to probe the surface of sterile containers 48 hours post-acquisition of X. f. piercei. The saliva deposited during attempted feeding probes was found to contain X. f. piercei. We observed no correlation between X. f. piercei titers in the foregut of H. vitripennis that fed on Xylella-infected grapevines and the presence of this bacterium in the deposited saliva. The infection rate after a 48 h post-acquisition feeding on healthy citrus and grapevines was observed to be 77% for H. vitripennis that fed on grapevines and 81% for H. vitripennis that fed on citrus, with no difference in the number of positive probing sites from H. vitripennis that fed on either grapevine or citrus. This method is amenable for individual assessment of X. f. piercei-infectivity, with samples less likely to be affected by tissue contamination that is usually present in whole body extracts. en
dc.description.provenance Submitted by Dorothea Salo (dsalo@library.wisc.edu) on 2008-04-07T14:26:12Z No. of bitstreams: 2 i1536-2442-8-Ramirez-v02.mov: 17048504 bytes, checksum: 284aaa1ed291953f333e8ebe38433830 (MD5) i1536-2442-8-Ramirez-v01.mov: 17260258 bytes, checksum: 8aeb592c8e88baeef95816cbae9eb232 (MD5) en
dc.description.provenance Approved for entry into archive by Dorothea Salo(dsalo@library.wisc.edu) on 2008-04-07T14:27:48Z (GMT) No. of bitstreams: 2 i1536-2442-8-Ramirez-v02.mov: 17048504 bytes, checksum: 284aaa1ed291953f333e8ebe38433830 (MD5) i1536-2442-8-Ramirez-v01.mov: 17260258 bytes, checksum: 8aeb592c8e88baeef95816cbae9eb232 (MD5) en
dc.description.provenance Made available in DSpace on 2008-04-07T14:27:48Z (GMT). No. of bitstreams: 2 i1536-2442-8-Ramirez-v02.mov: 17048504 bytes, checksum: 284aaa1ed291953f333e8ebe38433830 (MD5) i1536-2442-8-Ramirez-v01.mov: 17260258 bytes, checksum: 8aeb592c8e88baeef95816cbae9eb232 (MD5) Previous issue date: 2008 en
dc.format.extent 17048504 bytes
dc.format.extent 17260258 bytes
dc.format.mimetype video/quicktime
dc.format.mimetype video/quicktime
dc.subject H. vitripennis probing en
dc.subject sharpshooter feeding en
dc.subject plant pathogen transmission en
dc.title Detection of the bacterium, Xylella fastidiosa, in saliva of glassy-winged sharpshooter, Homalodisca vitripennis en
dc.type Video en

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