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Rieske Iron-Sulfur Isoforms in a Unicellular Cyanobacterium

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dc.contributor.advisor Kallas, Toivo
dc.contributor.author Schettle, Sarah
dc.date.accessioned 2007-12-18T20:28:19Z
dc.date.available 2007-12-18T20:28:19Z
dc.date.issued 2007-12-18T20:28:19Z
dc.identifier.uri http://digital.library.wisc.edu/1793/22345
dc.description Oshkosh Scholar, Volume 2, 2007, p. 81-89. en
dc.description.abstract Rieske iron-sulfur proteins (ISPs) are integrally involved in photosynthetic electron transport chains and thus closely linked to energy production and its regulation in cyanobacteria and plant chloroplasts. The cyanobacterium Synechocystis sp. PCC 6803 has three Rieske proteins, PetC1, PetC2, and PetC3, whose specific roles are not well understood. Here we describe two-dimensional (2D) gel electrophoresis and immunoblotting used to investigate the PetC1 Rieske protein in native Synechocystis and to confirm its absence in a mutant with an inactivated PetC1 gene. These studies revealed a “trail” of PetC1 Rieske protein spots migrating at the same molecular weight on 2D gels but at different isoelectric points. This “stuttering” phenomenon suggests covalent modifications that may reflect oxidative damage or modifications of the protein involved in biochemical signaling reactions. en
dc.description.provenance Submitted by Debra Duncan (duncan@uwosh.edu) on 2007-12-18T20:17:45Z No. of bitstreams: 1 rieske.pdf: 389235 bytes, checksum: 840f2c9ccf2ee6b644b24ea256c6c297 (MD5) en
dc.description.provenance Approved for entry into archive by Debra Duncan(duncan@uwosh.edu) on 2007-12-18T20:28:19Z (GMT) No. of bitstreams: 1 rieske.pdf: 389235 bytes, checksum: 840f2c9ccf2ee6b644b24ea256c6c297 (MD5) en
dc.description.provenance Made available in DSpace on 2007-12-18T20:28:19Z (GMT). No. of bitstreams: 1 rieske.pdf: 389235 bytes, checksum: 840f2c9ccf2ee6b644b24ea256c6c297 (MD5) en
dc.format.extent 389235 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.subject Chloroplasts en
dc.subject Plants, Effect of light on. en
dc.subject Photosynthesis en
dc.subject Electron transport en
dc.subject Cyanobacteria en
dc.title Rieske Iron-Sulfur Isoforms in a Unicellular Cyanobacterium en
dc.type Article en

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