http://digital.library.wisc.edu/1793/7951 http://minds.wisconsin.edu/retrieve/7803 http://digital.library.wisc.edu/1793/7951 Search the Channel search http://minds.wisconsin.edu/simple-search http://digital.library.wisc.edu/1793/23667 Title: Delta-opioid receptor (DOR) activation prolongs respiratory motor output during oxygen-glucose deprivation (OGD) in neonatal rat spinal cord in vitro <br/> <br/>Authors: Freiberg, Sara M. <br/> <br/>Abstract: Severe reduction of blood flow to the brain results in oxygen-glucose deprivation (OGD) and neuronal damage. DOR activation is neuroprotective during OGD in the cerebellum and cortex, but it is not known whether DOR activation protects spinal motor circuits that contribute to breathing. To address this question, a split-bath in vitro neonatal rat brainstem/spinal cord preparation was used to record spontaneous respiratory motor output from cervical (C4-C5) and thoracic (T4-T7) ventral spinal roots. A plastic barrier at spinal segment C1 allowed oxygenated solution to continuously bathe the brainstem while the solution on the spinal cord was switched from oxygenated to OGD (0 mM glucose, bubbled with 95% nitrogen/5% carbon dioxide) with or without DADLE (1.0 uM, DOR agonist). After OGD solution was applied to the spinal cord (n=7), cervical and thoracic motor output was abolished at 25.8 ± 2.0 and 26.4 ± 1.6 min, respectively. However, when DADLE was applied 10 min prior to and during spinal OGD solution application (n=8), cervical and thoracic motor output was abolished at 41.5 ± 4.9 min (p = 0.014) and 57.0 ± 4.2 min (p = <0.001), respectively. These data suggest that spinal DOR activation protects both cervical and thoracic respiratory motor output during OGD. <br/> <br/>Description: 2 p. (abstract and cover sheet only). YOU CANNOT OPEN THE COMPLETE PAPER. It is not available to the public, in accordance with the author’s wishes http://digital.library.wisc.edu/1793/23547 Title: Gaze-evoked blinks in rhesus monkeys <br/> <br/>Authors: Hintz, Adam S. <br/> <br/>Abstract: Gaze-evoked blinks, blinks that occur during rapid movements of the head and eyes, are believed to be the result of a shared mechanism that is responsible for a blink occurring with a saccade. In humans, it has been found that blinks are more likely to occur with larger saccades. Saccades accompanied with a blink are also more likely when attentional demands are low. Saccades associated with a reflex blink elicited by an air puff in monkeys have been shown to be slower than saccades without a blink. The purpose of the present study was to see if monkeys tested on visually-guided and memory-guided saccade tasks would show the same behavior in gaze-evoked blinks as humans. In this study, similar findings were found of gaze-evoked blinks in monkeys as were found in humans. Gaze-evoked blinks also slowed saccades as was found in reflex blinks elicited by a puff of air. <br/> <br/>Description: 13 p. http://digital.library.wisc.edu/1793/8152 Title: Determining the impacts of residential lakeshore development and various physical factors on largemouth bass (Micropterus salmoides) growth rates <br/> <br/>Authors: Guarascio, Matthew J. <br/> <br/>Abstract: Development of residential lakeshore properties can have several effects on aquatic ecosystems including the thinning of littoral coarse woody habitat. Previous studies have shown that reduction of littoral coarse woody habitat negatively affects largemouth bass (Micropterus salmoides) growth rates. In order to validate these results and attempt to find the mechanism through which bass growth rates are reduced, we surveyed 16 lakes in Vilas, County Wisconsin on a gradient of conductivity and development. Regressions of size-specific growth rates with lake area (p < .01) and maximum depth (p < .05) were negatively correlated and significant. A negative correlation was observed between mean largemouth bass (Micropterus salmoides) growth rate and development, between lake categories of low development low conductivity and high development high conductivity, although this trend was not statistically significant. Our research confirms that extensive residential lakeshore development may reduce the growth rates of bass as demonstrated by Schindler (2000). <br/> <br/>Description: 21 p. http://digital.library.wisc.edu/1793/8150 Title: Studies on the heat stability of egg yolk antibody (lgY) <br/> <br/>Authors: Bobeck, Elizabeth A. <br/> <br/>Abstract: Nutritionally and economically-important heat-labile proteins, including enzymes, hormones, and antibodies (Ab), lose a substantial amount of activity following industrial processing. Protection of these heat-sensitive bioactive molecules is needed in order to realize expanded markets for these biologics. Using a model of heat-labile proteins, Ab to phospholipase A2, and a sensitive detection system for Ab binding (ELISA), a pilot steam chamber was designed and constructed to develop methods of encapsulating proteins. After modification of the pilot chamber, it was shown that water plays a key role in Ab destruction. Samples (Ab, trehalose-encapsulated Ab, and industry standard) dried with drierite before steam treatment retained 100% activity after 60 seconds in 92-93C in a sealed 15ml centrifuge tube, while samples not dried but sealed prior to steam treatment lost activity (Ab retained 72.24% activity, trehalose-encapsulated Ab retained 74.03% activity, and industry standard retained 42.26% activity). A hydrophobic protein matrix (HPM) was developed. 41.73% binding activity remained in 1% Ab in pasta matrix, 0.94% remained in Ab in egg matrix, and 4.5% remained in industry standard after 60s in 92-93C in unsealed containers. This RPM may protect against steam-induced losses by protecting Ab from water. <br/> <br/>Description: 59 p.